Electron microscopy for 3D reconstructions

Learn Electron Microscopy for 3D reconstructions of serial sections

image and post-image processing course

In just 2 days

Learn how to reconstruct 3D structures from TEM images. You’ll get detailed information on volume and area as well as more thorough information on morphology.

In this 2-day training course you’ll learn how to perform 3D reconstruction of a series of TEM images acquired with a standard TEM.

What you will learn in this course

By the end of this course you’ll be able to:

  • Acquire images from series of sections
  • Montage and align series of images
  • Reconstruct selected structure
  • Handle the data output and create a video

Basic knowledge of TEM sample prep and imaging will be needed for this course.

But don’t worry if you need to learn that too, we can tailor made the right training package that includes all the necessary skills that you need.

You can also bring your own sample, and get some data out of it at the same time with 3D reconstruction of a region of your choice.

Why Choose Us

Hands-on individual training

All our courses are mostly individually run – only then you get the benefit of having a lot of hands on practice and we can ensure the training is perfectly suited to your needs – including the samples and microscope conditions you’re most likely to need.

During the training, we will demonstrate to you the necessary steps involved in TEM imaging and processing of the images. You will then repeat the procedures until you feel comfortable with them.

At the course completion you will receive a certificate of attendance.

The outline of activities/skills you’ll learn



Alignment necessary for serial imaging


Set up automated imaging

Operating and setting up SerialEM software



Practice of setting up SerialEM software for imaging


Handling images

8-bit transform, aberration correction, montaging, alignment + practise on another set of images


3D reconstruction

of a region of choice, plus practice if necessary


Data analysis

Making sense of the data, creating a video and anything else you may need

Interested in the course?

Contact us now