Sample preparation for TEM course
Learn to prepare biological samples
Learn the complexities of biological TEM sample preparation with our comprehensive course. Designed to cater to your specific needs, whether that is using cell cultures (pellets or adhered cells) or tissues for TEM.
Depending on your requirements, you may also choose to gain expertise on negative staining, perfect for imaging viruses, phage, liposomes, and exosomes.
Master all aspects of sample preparation
From start to finish, we cover all aspects of sample preparation. Learn the essential considerations for preparing your samples, ensuring the best possible outcome. The laboratory process encompasses chemical fixation, dehydration, and resin infiltration, resulting in epoxy resin blocks.
Gain expertise in block sectioning using both glass and diamond knives, mastering the art of handling a microtome. You’ll also discover how to collect samples onto grids and acquire essential skills in basic grid preparation. Lastly, we’ll teach you how to counter-stain samples with uranyl acetate and lead citrate.
For researchers and technical staff
This course presents an excellent opportunity for researchers and technical staff seeking to broaden their skill set and excel in TEM sample preparation.
What you’ll learn in this course:
| Activity |
| Sample selection and various approaches |
| Fixation (glutaraldehyde and osmium) |
| dehydration |
| Embedding demonstration (resin prep, 2 changes to infiltrate, placing into a mould) |
| Preparing sample block (with razor blade, glass knife) |
| Practice preparing a block |
| Microsectioning |
| Practice of microsectioning |
| Grid preparation – Support films |
| Staining of final slots |
| Preparation of nanoparticles for TEM (drying on grids) |
| Negative staining demonstration +practice |
Ready to finally become proficient in preparing biologicals for TEM?
Enrol now in our TEM sample preparation course and master biological sample preparation.
